Generation of Live Attenuated Novel Influenza Virus A/California/7/09 (H1N1) Vaccines with High Yield in Embryonated Chicken Eggs
Identifieur interne : 000F90 ( Main/Exploration ); précédent : 000F89; suivant : 000F91Generation of Live Attenuated Novel Influenza Virus A/California/7/09 (H1N1) Vaccines with High Yield in Embryonated Chicken Eggs
Auteurs : ZHONGYING CHEN [États-Unis] ; WEIJIA WANG [États-Unis] ; HELEN ZHOU [États-Unis] ; Amorsolo L. Jr Suguitan [États-Unis] ; Cindy Shambaugh [États-Unis] ; Lomi Kim [États-Unis] ; Jackie Zhao [États-Unis] ; George Kemble [États-Unis] ; HONG JIN [États-Unis]Source :
- Journal of virology [ 0022-538X ] ; 2010.
Descripteurs français
- Pascal (Inist)
- Wicri :
- topic : Vaccin.
English descriptors
- KwdEn :
Abstract
Several live attenuated influenza virus A/California/7/09 (H1N1) (CA09) candidate vaccine variants that possess the hemagglutinin (HA) and neuraminidase (NA) gene segments from the CA09 virus and six internal protein gene segments from the cold-adapted influenza virus A/Ann Arbor/6/60 (H2N2) virus were generated by reverse genetics. The reassortant viruses replicated relatively poorly in embryonated chicken eggs. To improve virus growth in eggs, reassortants expressing the HA and NA of CA09 were passaged in MDCK cells and variants exhibiting large-plaque morphology were isolated. These variants replicated at levels approximately 10-fold higher than the rate of replication of the parental strains in embryonated chicken eggs. Sequence analysis indicated that single amino acid changes at positions 119, 153, 154, and 186 were responsible for the improved growth properties in MDCK cells and eggs. In addition, the introduction of a mutation at residue 155 that was previously shown to enhance the replication of a 1976 swine influenza virus also significantly improved the replication of the CA09 virus in eggs. Each variant was further evaluated for receptor binding preference, antigenicity, attenuation phenotype, and immunogenicity. Mutations at residues 153, 154, and 155 drastically reduced viral antigenicity, which made these mutants unsuitable as vaccine candidates. However, changes at residues 119 and 186 did not affect virus antigenicity or immunogenicity, justifying their inclusion in live attenuated vaccine candidates to protect against the currently circulating 2009 swine origin H1N1 viruses.
Affiliations:
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Influenza Virus A/California/7/09 (H1N1) Vaccines with High Yield in Embryonated Chicken Eggs</title><author><name sortKey="Zhongying Chen" sort="Zhongying Chen" uniqKey="Zhongying Chen" last="Zhongying Chen">ZHONGYING CHEN</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>MedImmune, 319 North Bernardo Ave.</s1><s2>Mountain View, California 94043</s2><s3>USA</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ></inist:fA14><country>États-Unis</country><wicri:noRegion>Mountain View, California 94043</wicri:noRegion></affiliation></author><author><name sortKey="Weijia Wang" sort="Weijia Wang" uniqKey="Weijia Wang" last="Weijia Wang">WEIJIA WANG</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>MedImmune, 319 North Bernardo Ave.</s1><s2>Mountain View, California 94043</s2><s3>USA</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ></inist:fA14><country>États-Unis</country><wicri:noRegion>Mountain View, California 94043</wicri:noRegion></affiliation></author><author><name sortKey="Helen Zhou" sort="Helen Zhou" uniqKey="Helen Zhou" last="Helen Zhou">HELEN ZHOU</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>MedImmune, 319 North Bernardo Ave.</s1><s2>Mountain View, California 94043</s2><s3>USA</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ></inist:fA14><country>États-Unis</country><wicri:noRegion>Mountain View, California 94043</wicri:noRegion></affiliation></author><author><name sortKey="Suguitan, Amorsolo L Jr" sort="Suguitan, Amorsolo L Jr" uniqKey="Suguitan A" first="Amorsolo L. Jr" last="Suguitan">Amorsolo L. Jr Suguitan</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>MedImmune, 319 North Bernardo Ave.</s1><s2>Mountain View, California 94043</s2><s3>USA</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ></inist:fA14><country>États-Unis</country><wicri:noRegion>Mountain View, California 94043</wicri:noRegion></affiliation></author><author><name sortKey="Shambaugh, Cindy" sort="Shambaugh, Cindy" uniqKey="Shambaugh C" first="Cindy" last="Shambaugh">Cindy Shambaugh</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>MedImmune, 319 North Bernardo Ave.</s1><s2>Mountain View, California 94043</s2><s3>USA</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ></inist:fA14><country>États-Unis</country><wicri:noRegion>Mountain View, California 94043</wicri:noRegion></affiliation></author><author><name sortKey="Kim, Lomi" sort="Kim, Lomi" uniqKey="Kim L" first="Lomi" last="Kim">Lomi Kim</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>MedImmune, 319 North Bernardo Ave.</s1><s2>Mountain View, California 94043</s2><s3>USA</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ></inist:fA14><country>États-Unis</country><wicri:noRegion>Mountain View, California 94043</wicri:noRegion></affiliation></author><author><name sortKey="Zhao, Jackie" sort="Zhao, Jackie" uniqKey="Zhao J" first="Jackie" last="Zhao">Jackie Zhao</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>MedImmune, 319 North Bernardo Ave.</s1><s2>Mountain View, California 94043</s2><s3>USA</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ></inist:fA14><country>États-Unis</country><wicri:noRegion>Mountain View, California 94043</wicri:noRegion></affiliation></author><author><name sortKey="Kemble, George" sort="Kemble, George" uniqKey="Kemble G" first="George" last="Kemble">George Kemble</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>MedImmune, 319 North Bernardo Ave.</s1><s2>Mountain View, California 94043</s2><s3>USA</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ></inist:fA14><country>États-Unis</country><wicri:noRegion>Mountain View, California 94043</wicri:noRegion></affiliation></author><author><name sortKey="Hong Jin" sort="Hong Jin" uniqKey="Hong Jin" last="Hong Jin">HONG JIN</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>MedImmune, 319 North Bernardo Ave.</s1><s2>Mountain View, California 94043</s2><s3>USA</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ></inist:fA14><country>États-Unis</country><wicri:noRegion>Mountain View, California 94043</wicri:noRegion></affiliation></author></analytic><series><title level="j" type="main">Journal of virology</title><title level="j" type="abbreviated">J. virol.</title><idno type="ISSN">0022-538X</idno><imprint><date when="2010">2010</date></imprint></series></biblStruct></sourceDesc><seriesStmt><title level="j" type="main">Journal of virology</title><title level="j" type="abbreviated">J. virol.</title><idno type="ISSN">0022-538X</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Attenuated strain</term><term>California</term><term>Chicken</term><term>Influenza A virus</term><term>Vaccine</term><term>Yield</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Virus grippal A</term><term>Poulet</term><term>Souche atténuée</term><term>Californie</term><term>Vaccin</term><term>Rendement</term><term>Influenzavirus A(H1N1)</term></keywords><keywords scheme="Wicri" type="topic" xml:lang="fr"><term>Vaccin</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Several live attenuated influenza virus A/California/7/09 (H1N1) (CA09) candidate vaccine variants that possess the hemagglutinin (HA) and neuraminidase (NA) gene segments from the CA09 virus and six internal protein gene segments from the cold-adapted influenza virus A/Ann Arbor/6/60 (H2N2) virus were generated by reverse genetics. The reassortant viruses replicated relatively poorly in embryonated chicken eggs. To improve virus growth in eggs, reassortants expressing the HA and NA of CA09 were passaged in MDCK cells and variants exhibiting large-plaque morphology were isolated. These variants replicated at levels approximately 10-fold higher than the rate of replication of the parental strains in embryonated chicken eggs. Sequence analysis indicated that single amino acid changes at positions 119, 153, 154, and 186 were responsible for the improved growth properties in MDCK cells and eggs. In addition, the introduction of a mutation at residue 155 that was previously shown to enhance the replication of a 1976 swine influenza virus also significantly improved the replication of the CA09 virus in eggs. Each variant was further evaluated for receptor binding preference, antigenicity, attenuation phenotype, and immunogenicity. Mutations at residues 153, 154, and 155 drastically reduced viral antigenicity, which made these mutants unsuitable as vaccine candidates. However, changes at residues 119 and 186 did not affect virus antigenicity or immunogenicity, justifying their inclusion in live attenuated vaccine candidates to protect against the currently circulating 2009 swine origin H1N1 viruses.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country></list><tree><country name="États-Unis"><noRegion><name sortKey="Zhongying Chen" sort="Zhongying Chen" uniqKey="Zhongying Chen" last="Zhongying Chen">ZHONGYING CHEN</name></noRegion><name sortKey="Helen Zhou" sort="Helen Zhou" uniqKey="Helen Zhou" last="Helen Zhou">HELEN ZHOU</name><name sortKey="Hong Jin" sort="Hong Jin" uniqKey="Hong Jin" last="Hong Jin">HONG JIN</name><name sortKey="Kemble, George" sort="Kemble, George" uniqKey="Kemble G" first="George" last="Kemble">George Kemble</name><name sortKey="Kim, Lomi" sort="Kim, Lomi" uniqKey="Kim L" first="Lomi" last="Kim">Lomi Kim</name><name sortKey="Shambaugh, Cindy" sort="Shambaugh, Cindy" uniqKey="Shambaugh C" first="Cindy" last="Shambaugh">Cindy Shambaugh</name><name sortKey="Suguitan, Amorsolo L Jr" sort="Suguitan, Amorsolo L Jr" uniqKey="Suguitan A" first="Amorsolo L. Jr" last="Suguitan">Amorsolo L. Jr Suguitan</name><name sortKey="Weijia Wang" sort="Weijia Wang" uniqKey="Weijia Wang" last="Weijia Wang">WEIJIA WANG</name><name sortKey="Zhao, Jackie" sort="Zhao, Jackie" uniqKey="Zhao J" first="Jackie" last="Zhao">Jackie Zhao</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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